Image of the Month

Damavand, the highest Asian inactive volcano in the Alborz mountains, around 66 km in the northeastern Tehran, Iran (the highest elevation is 5,671 m) (Photo by M. H. Azizi MD, Winter 2015).

A winter view of Abali, around 57 km in the northeastern Tehran, Iran (Photo by M. H. Azizi MD, 2015).


Announcement

The collection of articles appearing in the current book consists of manuscripts on the history of medicine in Iran, initially published from 1998 to 2014 in the “Archives of Iranian Medicine” (AIM). These articles are categorized into four separate parts, based on their subjects: Part 1) Ancient Times, Medieval Period, Part 2) Contemporary Medicine, Part 3) Outstanding Physicians and Part 4) Historical Background of Fatal Diseases in Iran. These 73 manuscripts have now been compiled in a 446 pages book for those who are interested in the history of Iranian medicine.more

Since the Archive of Iranian Medicine (AIM) journal presents as Open Access monthly, online periodical from May 2013, thus dear readers may refer to the journal website (www.aimjournal.ir) for free downloading of the published papers.

AIM Office


About Us

A Monthly Peer-Reviewed Medical Journal Published by the Academy of Medical Sciences of the I.R. Iran; Indexed in PubMed/MEDLINE, ISI Web of Science, EMBASE, SCOPUS, CINHAL, PASCAL, CSA, SID, ISSN: Print 1029-2977, Online 1735-3947.The impact factor of Archives of Iranian Medicine according to Journal Citation Reports®(JCR®) 2012 is 1.222.

Announcement
Dear Readers;

The Archives of Iranian Medicine Journal will not accept case reports from March 2015 until December 2015.

With best regards
AIM Office
  
Selected Article
Multipotent SSEA-1 Positive Cells Population Differentiation into Primordial Germ Cells and Subsequently Progress into Oocyte-like Cells 1

Authors’ affiliations: 1Cellular and Molecular Research Center, Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran, 2Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

•Corresponding author and reprints: Maryam Nazm Bojnordi MD PhD, Cellular and Molecular Research Center, Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Tel: +98-151-3543081(2429), Fax: +98-151-3543086, E-mail: bojnordi@modares.ac.ir. 

Accepted for publication: 27 May 2015

Pubmedxml   Download PDF Fulltext
Authors: Hatef Ghasemi Hamidabadi1, Aligholi Sobhani2, *Maryam Nazm Bojnordi3,
 
Keywords: BMP4, conditioned medium, embryoid bodies, SSEA-1
BACKGROUND: Stage-specific embryonic antigen- 1 (SSEA1) is a cell surface carbohydrate that its pattern expression is changed during induction of mouse embryonic stem cell differentiation. In this study, the spatial distribution of SSEA1 on primordial germ cells differentiation and subsequent progression into oocyte-like cells from mouse embryonic stem cells in vitro was evaluated.
METHODS: Embryoid bodies from mouse embryonic stem cells were cultured for two days with 5 ng/mL BMP4. SSEA-1 positive and negative cells were separated using the MACS system and cultured separately in a conditioned medium consist of in vitro maturation medium diluted in DMEM [1:1] for 10 days. We assayed viability, colony formation and alkaline phosphatase activity (ALP) of sorted cells. Also, germ cell markers were analyzed by flow cytometry, Immunocytochemistry and RT-PCR.
RESULTS: Viability percent SSEA-1 positive cells were more than SSEA-1 negative cells. SSEA-1 positive cells and SSEA-1 negative cells formed compact and flat colonies respectively. Unlike the SSEA-1 positive population, the SSEA-1 negative colonies showed a weak ALP activity. SSEA-1 positive cells expressed Oct4, Stella, Mvh, c-kit, Scp3, Desmin, GFAP and Albumin. Interestingly, SSEA-1 negative cells expressed Desmin and GFAP. The population of Mvh-positive cells in SSEA-1 positive was 17.74%. All specific oocyte mentioned genes were detected in the SSEA-1 positive. Also, oocyte specific proteins GDF9 and ZP3 were detected using Immunocytochemistry.
CONCLUSION: Our results suggest that conditioned medium provides a suitable niche to differentiation and progression putative primordial germ cells derived from the SSEA-1positive toward oocyte-like cells.
 
ISSN:1029-2977       Article type:Original
  
Upcoming Events
  
Useful Links
  
Privacy Statement    |    Terms Of Use Copyright 2009 by Academy of Medical Sciences of the I.R. Iran