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Arch Iran Med. 2021;24(8): 607-614.
doi: 10.34172/aim.2021.86
  Abstract View: 314
  PDF Download: 218

Original Article

Platelet Microparticles Accelerate Proliferation and Growth of Mesenchymal Stem Cells through Longevity-Related Genes

Maryam Samareh Salavati Pour 1,2,3 ORCID logo, Fatemeh Hoseinpour Kasgari 3, Alireza Farsinejad 2,3, Ahmad Fatemi 3, Gholamhossein Hassanshahi 4, Roohollah Mirzaee Khalilabadi 3* ORCID logo

1 Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran
2 Cell Therapy and Regenerative Medicine Comprehensive Center, Kerman University of Medical Sciences, Kerman, Iran
3 Department of Hematology and Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran
4 Department of Immunology, Medical School, Rafsanjan University of Medical Sciences and Health Services, Rafsanjan, Iran
*Corresponding Author: Roohollah Mirzaee Khalilabadi, PhD, Kerman University of Medical Sciences, Medical University Campus, Haft-Bagh Highway, Kerman, Iran. Tel: +98-9133904227; Fax: 034-31325375; Email: . khalilabadi60@gmail.com

Abstract

Background: Due to their self-renewal and differentiation ability, the mesenchymal stem cells (MSCs) have been studied extensively. However, the MSCs lifespan is restricted; they undergo several divisions in vitro that cause several alternations in cellular features and relatively lessens their application. Thus, this study was aimed to assess the effect of platelet-derived microparticles (PMPs), a valuable source of proteins, microRNAs (miRNAs), and growth factors, on the expression of hTERT, c-MYC, p16, p53, and p21 as the most important aging and cell longevity genes alongside with population doubling time (PDT) of PMP-treated cells in comparison to a control group.

Methods: Umbilical cord MSCs (UC-MSCs) were used in this study, whereby they reached a confluency of 30%. MSCs were treated by PMPs (50 µg/mL), and then, PDT was determined for both groups. Quantitative expression of hTERT, c-MYC, p16, p53, and p21 was examined through quantitative real-time PCR at various intervals (i.e. after five and thirty days as well as freezing-thawing process).

Results: Our results demonstrated that the treated group had a shorter PDT in comparison to the control group (P<0.050). The real-Time PCR data also indicated that PMPs were able to remarkably up-regulate hTERT and c-MYC genes expression while down-regulating the expression of p16, p21, and p53 genes (P<0.050), especially following five days of treatment.

Conclusion: According to these data, it appears that PMPs are a safe and effective candidate for prolonging the lifespan of UC-MSCs; however, further investigations are needed to corroborate this finding.


Keywords: Aging, Blood platelets, Cell-derived microparticle, Gene expression, Mesenchymal stem cell
Cite this article as: Salavati Pour MS, Hoseinpour Kasgari F, Farsinejad A, Fatemi A, Hassanshahi G, Mirzaee Khalilabadi R. Platelet microparticles accelerate proliferation and growth of mesenchymal stem cells through longevity-related genes. Arch Iran Med. 2021;24(8):607-614. doi: 10.34172/aim.2021.86
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Submitted: 04 Jan 2020
Accepted: 30 Sep 2020
ePublished: 01 Aug 2021
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